Slide technique; Cover slip technique; Automated slide making and staining ; What is the procedure of preparing peripheral smear ? A second glass slide is used as a spreader, streaking the blood into a thin film across the glass slide. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. There are different staining techniques that are available in the bacteriology laboratory. Peripheral Blood Smear. ICSH has published a “reference” staining method for blood films based on purified azure B and eosin Y solutions (ICHS, 2010). A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. They appear as biconcave discs of uniform shape and size (7.2 microns) that lack organelles and granules. Made with ♡ by Sagar Aryal. Steps of microbial smear preparation : Types of Stains 7. Smear technique. The are two additional types of blood smear used for specific purposes 1. 2. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type… Aim for a blood droplet size of 4mm diameter. ing and then drying before staining. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. Neutrophils are displayed as a deep blue/purple nucleus under the microscope. Clinical relevance of this analysis and its interpretation are very important. Use the “squash” technique illustrated above for mucoid or viscous fluids (tracheal wash, joint fluid, bile). The basic one-step smearing technique (Abele et al, 1985) is designed to process a harvest consisting of one or two droplets of semisolid tissue material. Mismatched components/mechanisms on your microscope may also be a contributing factor. Dmitri Leonidovich Romanowsky later modified Ehrlich's stain to produce a wider range of colours, creating the Romanowsky stain, which is still used to stain blood smears … Some additional guidelines should be followed to create the best blood smear… Blood films are typically stained by Romanowsky dyes (consisting of a variety of thiazines and eosins) (Henry, 1998). Quantitative importance enables the numbering of blood cells while the qualitative function is to demonstrate and identify the cell morphologies, including types of leukocytes, erythrocytes, monocytes, and platelets. Perhaps there is a problem with the optics and magnifications. WEDGE BLOOD SMEAR • Specimen: Peripheral blood smear made from EDTA- anticoagulated blood. MicroscopeMaster.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means to earn fees by linking to Amazon.com and affiliated sites. 30×) than in an equal area of a thin smear. 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